Effect of Gossypol, Apogossypol and Gossypolone on Fatty Acids Pattern of Lipid Fractions in Rat Serum and Seminal Fluid

The fatty acids pattern of the total lipid fractions of male rat serum and seminal fluids were studied under the effect of gossypol and some of its metabolites (Apogossypol and gossypolone). The applied dose of gossypol and its derivatives exhibits some metabolic effects such as significant decrease of the saturated fatty acids and a significant increase of polyunsaturated fatty acids. In the serum, eicosapentaenoic acid (C20:5) in sterylester fraction was significantly increased under the effect of apogossypol. While in the free fatty acids fraction, palmitic acid (Cl6:0) content was significantly decreased, meanwhile significant increase of polyunsaturated fatty acid (C22:5) with apogossypol and gossypolone treatment. Stearic acid (Cl8:0) was significantly decreased in the total phospholipids fraction due to the treatment with gossypol acetic acid, apogossypol and gossypolone. In the seminal fluid, also eicosapentaenoic acid (C20:5), in sterylester fraction, was significantly increased with apogossypol treatment. In triglycerides, free fatty acids and phospholipids fractions, mono-unsaturated fatty acids were significantly decreased after gossypol and gossypolone treatment. Also, linoleic acid was significantly increased in the main fractions (triglycerides) under the effect of gossypol and gossypolone. Gossypol and gossypolone which induced sterility, may have significant effect on NAD and NADP-dependent enzymes, ATPase and/or have local effect on the germinal cells. Therefore, apogossypol which did not cause sterility, has no effect on the process of spermatogenesis as well as the content of seminal monounsaturated fatty acids.